The role of deoxyribonucleic acid in ribonucleic acid synthesis. I. The purification and properties of ribonucleic acid polymerase.

The influence of syringomycin on ribonucleic acid synthesis.

Syringomycin, a wide-spectrum antibiotic produced by strains of Pseudomonas syringae which cause bacterial canker of peach, was able to bind to salmon sperm and calf thymus deoxyribonucleic acid but not to calf thymus histone; it also inhibited ribonucleic acid polymerase activity. These abilities to bind to deoxyribonucleic acid and to inhibit ribonucleic acid polymerase were inactivated when ...

Template requirement of maize RNA polymerase.

Maize RNA polymerase utilizes heated deoxyribonucleic acid more effectively than native deoxyribonucleic acid as a template for ribonucleic acid synthesis. A ribonucleic acid-deoxyribonucleic acid hybrid accumulates in the presence of heated deoxyribonucleic acid. The amount of product formed with either native or heat-denatured deoxyribonucleic acid does not exceed the amount of deoxyribonucle...

Enzymatic Synthesis of Ribonucleic Acid. Iv. the Deoxyribonucleic Acid-directed Synthesis of Complementary Cytoplasmic Ribonucleic Acid Components.

The ribonucleic acid product formed by ribonucleic acid polymerase resembles its deoxyribonucleic acid template in base composition and in base sequence (l-6). The reaction in vitro results in the synthesis of RNA chains which are complementary to both DNA strands (6). Evidence for this originates from the formation of highly ordered RNA structures when the RNA polymerase product is annealed wi...

Indirect evidence for the presence of Ribonucleic acid in vesicular stomatitis Virions

The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid V. PURIFICATION AND PROPERTIES OF THE DEOXYRIBONUCLEIC ACID-METHYLATING ACTIVITY OF ESCHERICHIA COLP

The classical investigations of Wyatt (2) and Dunn and Smith (3) established the existence of 5-methylcytosine and 6-methylaminopurine as constituents of the deoxyribonucleic acid of higher plants and animals, and of bacteria and bacterial viruses, respectively. Until recently, the biochemical pathways for the incorporation of these “trace bases” into deoxyribonucleic acid was unknown although ...